introduction

<aside> ⚡ Method for separating large molecules based on their size, such as DNA, RNA and proteins

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• Relies on the fact that DNA molecules have a negative charge due to its phosphate groups
• Gel made of agarose (polysaccharide polymer)
  • Comes from seaweed
  • Lets the DNA molecules travel within it
  • DNA is often suspended in a solution with dye

how does it work?

• wells
• restriction enzymes
• speed of DNA
• DNA bands
• DNA ladders
• steps involved

useful applications

• DNA fingerprinting
• southern blotting
• classification of organisms